Protein expression, purification, and proteomics core

  1. Biophysical characterization of purified proteins
  2. Construction of fusion tagged proteins either chromosomal or using exogenous plasmid vectors.
  3. Affinity purification using protein specific antibody or epitope tags fused with bait proteins of interest to identify interacting partners through mass spectrometry
  4. Offer range of chromatography separation techniques:
    1. Affinity Chromatography
    2. Ion Exchange Chromatography
    3. Size Exclusion Chromatography
    4. Hydrophobic Interaction Chromatography
    5. Reversed Phase Chromatography
  5. Sample preparation (desalting, digestion, and fractionation)
  6. Custom packed LC column services (based on the user needs) for any chromatography separation type and dimension.
  7. Determination of intact proteins and protein identification from silver-stain or SDS-PAGE gels.
  8. Protein identification in complex mixtures using a combination of easy Nano-LC and high-resolution mass spectrometer workflow.
  9. Relative protein quantification based on label-fee, SILAC, or iTRAQ
  10. Identification and quantification of proteins, peptides, and post-translational modifications (through TiO2 and IMAC).
  11. Mass spectrometry based proteomics and bioinformatics analysis (to identify reliable interacting proteins using database search engines) includes:
    1. SEQUEST
    3. MaxQuant
    4. Xtandem!
    5. MS-GF+
    6. and other data-processing and analysis tools such as Proteome Discoverer and Scaffold.
  12. For the protein interactions studies, the report will include scores assigned to each interacting protein pair to emphasize the significance and reliability of the interactions using algorithms such as
    1. purification enrichment score
    2. hypergeometric distribution
    3. COMPASS
    4. SAINT among others.