1. Using CRISPR/Cas9 technology we provide services to generate monoclonal or polyclonal gene-knockout of various cell lines of the desired gene of interest. We can also generate homo- or heterozygous gene knockout if needed
  2. We can also generate cell lines with reduction of expression of genes of interest using either stably-expressed shRNA or using CRISPR interference (CRISPRi, PMID 25307932) using third-generation lentiviral vectors in both cases.
  3. We can also provide genetically modified cell lines with increased expression of gene of interest of an exogenous gene/reporter. We can also generate cell lines with increased expression of the gene of interest from its endogenous genomic locus using CRISPR/Cas9-SAM (Synergistic Activation Mediator, PMID 25494202) with semi-tailored increase level of expression according to experimental need.
  4. Lastly, we can also provide cell lines of interest with chromosomally-edited desired gene of interest, whether by tagging the gene or by creating disease (or otherwise) gene mutations according to desired experimental paradigm.